Coding
DntR

Part:BBa_I723010:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-09-06)

DntR coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 828
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 439


Design Notes

This transcriptional regulator is encoded in the opposite direction to that of its target promoter.


Source

Cloned from a historical plasmid based on pQF52 carrying genomic sequences from Burkholderia cepacia.

References